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В биохимическом составе надземной части иван-чая присутствуют витамин С(этого антиоксиданта в кипрее в 3 раза больше чем в апельсинах и в 6,5 раза больше чем в лимонах), каротиноиды (предшественники витамина А), витамины группы B, слизи (полисарахиды), пектины, хлорофилл, дубильные вещества (до 20%), органические кислоты, фитостеролы (в том числе бета-ситостерин), тритерпеноиды, кумарины, флавоноиды (в том числе кверцетин и кемпферол), небольшое количество алкалоидов, а также некоторые макро- и микроэлементы (особенно высока концентрация в листьях и цветках иван-чая железа, меди и марганца, содержатся также в листьях и цветках кипрея в значительном количестве калий, натрий, кальций, магний, бор, никель и титан).

В богатых крахмалом, полисахаридами, легкоусваиваемыми белками и органическими кислотами корневищах иван-чая отсутствуют дубильные вещества, но содержатся в значительном количестве соли фосфора, кальция и кобальта.

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